Recombinant Protein Expression Services

The recombinant protein production process is often complicated and time-consuming. However, with the right expertise and a reliable partner, your protein production project can be successful. We offer a wide range of custom services that cover all the steps of recombinant protein production, from codon enhancement and gene synthesis to marginal testing and scale-up.

The first step in Recombinant Protein Expression Services protein production is the isolation of the gene that produces your protein of interest from human or animal cells in culture. The gene is then cloned into a DNA vector that will enable the gene to be expressed in a host cell. The expression vector also includes the promoter and ribosome binding site necessary for expression.

Traditionally, proteins are made by transfecting the expression host cells with a DNA vector containing the gene of your protein and then culturing them to express the gene and produce the recombinant protein. This approach is commonly used to make antibodies, reagents for immunochemistry, and other laboratory products. However, it is not always the best approach for recombinant protein production services in clinical studies, where higher levels of protein yield and purity are needed to meet strict pharmaceutical guidelines.

Choosing the Right Partner for Custom Antibody Services

There are four different protein expression systems that are frequently used in both research and industrial applications: bacteria, yeast, baculovirus/insect cells, and mammalian cells. Each system has advantages and disadvantages depending on the intended protein property, function, and downstream application. For example, bacterial systems are less expensive and faster to test than yeast, baculovirus/insect cell or mammalian systems, but they can produce insoluble inclusion bodies that can be difficult to purify.